WGA of Myxococcus xanthus natural isolates - SRA

SRX5168038: WGA of Myxococcus xanthus natural isolates
1 PACBIO_SMRT (PacBio RS II) run: 163,482 spots, 640.6M bases, 2.3Gb downloads

Design: A total of 10 g of gDNA were sheared to an average size distribution of 10 Kbp, using a gTube (Covaris , USA). A Bioanalyzer 2100 12K DNA Chip assay (Agilent, USA) was used to assess the fragment size distribution. A total of 5 g of sheared gDNA was DNA-damage repaired and end-repaired using polishing enzymes. A blunt-end ligation reaction followed by exonuclease treatment was performed to create the SMRT bell template. The library was quality inspected and quantified on the Agilent Bioanalyzer 12Kb DNA Chip and on a Qubit Fluorometer (Invitrogen, USA), respectively. A ready-to-use sequence SMRTbell-Polymerase Complex was created using the P4 DNA/Polymerase binding kit 2.0 (Pacific Biosciences, USA) according to the manufacturers instructions. The Pacific Biosciences RS2 instrument was programmed to load and sequence the samples on PacBio SMRT cells v3.0 (Pacific Biosciences, USA), taking one movie of 240 minutes each per SMRT cell. The MagBead (Pacific Biosciences, USA) loading method was chosen in order to improve the enrichment of longer fragments. After the run, a sequencing report was generated for every cell, via the SMRT portal, in order to assess the adapter dimer contamination, the sample loading efficiency, the obtained average read-length and the number of filtered sub-reads.

Submitted by: ETH Zurich

Study: Social genes are selection hotspots in kin groups of a soil microbe

PRJNA342411 • SRP173032 • All experiments • All runs

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The aim of this project is to assess the genetic diversity within single fruiting bodies of Myxococcus xanthus isolated from soil. Since relatedness in these multicellular structures is very high, whole genomes were sequenced, assembled and finished to a single contig for each of one individual within six fruiting bodies. The purpose of this study is to identify the evolutionary forces that drive differentiation in myxobacteria in soil.

Sample:

SAMN05757019 • SRS4121422 • All experiments • All runs

Organism: Myxococcus xanthus

Library:

Name: MXANTHUS_GH5.1.9c20.5

Instrument: PacBio RS II

Strategy: WGA

Source: GENOMIC

Selection: RANDOM

Layout: SINGLE

Runs: 1 run, 163,482 spots, 640.6M bases, 2.3Gb

Run	# of Spots	# of Bases	Size	Published
SRR8357320	163,482	640.6M	2.3Gb	2019-01-22

ID:: 6982650

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